Two simple, sensitive, accurate and precise spectrophotometric methods (method A and method B) have been developed and validated for the determination of hyoscine butylbromide. The first method involved reduction of ferric ions to ferrous ions and in turn formed coloured complex with potassium ferricyanide which showed absorption maxima at 725 nm. In the second method, hyoscine butylbromide in presence of acidic medium reacts with excess amount of chloramine-T and unreacted chloramine-T reacts with crystal violet to produce greenish-blue colour. The absorption maxima was found to be 598 nm. The linearity range was found to be in concentration range 5-25 µg/ml for both the methods. The correlation coefficient was found to be 0.9998 and 0.9996 for method A and method B respectively. The regression equation was found to be y = 0.0371x + 0.0045 and y = 0.1732x - 0.031 for method A and method B respectively. The method was validated according to ICH guidelines.
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